TIMD4 Protein (AA 25-305) (His tag)

Catalog No. ABIN7275733

This Recombinant TIMD4 protein is expressed in HEK-293 Cells.

Quick Overview for TIMD4 Protein (AA 25-305) (His tag) (ABIN7275733)

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305

Purification tag / Conjugate: This TIMD4 protein is labelled with His tag.

Purpose: Cynomolgus TIM-4 Protein

Sequence: Glu25-Gln305

Characteristics: Recombinant Cynomolgus TIM-4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Gln305.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus TIM-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus TIM-4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: TIM4

Background: Tim4 is a transmembrane protein as the phosphatidylserine (PS) receptor, known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed highly in macrophages, and macrophage Tim-4 inhibits inflammation under various conditions of immune activation.

Molecular Weight: 31.55 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005558436

Pathways: Cancer Immune Checkpoints