TNC Protein (AA 23-621) (His tag)

Catalog No. ABIN7275754

This Recombinant TNC protein is expressed in HEK-293 Cells.

Quick Overview for TNC Protein (AA 23-621) (His tag) (ABIN7275754)

Target: TNC (Tenascin C (TNC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-621

Purification tag / Conjugate: This TNC protein is labelled with His tag.

Purpose: Mouse Tenascin Protein

Sequence: Gly23-Ser621

Characteristics: Recombinant Mouse Tenascin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly23-Ser621.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Tenascin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Tenascin is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin

Background: Tenascin-C (TNC) is a hexameric, multimodular extracellular matrix protein with several molecular forms that are created through alternative splicing and protein modifications. It is highly conserved amongst vertebrates, and molecular phylogeny indicates that it evolved before fibronectin. Tenascin-C has many extracellular binding partners, including matrix components, soluble factors and pathogens, it also influences cell phenotype directly through interactions with cell surface receptors.

Molecular Weight: 65.65 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: Q80YX1

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development