TNFRSF1B Protein (AA 23-257) (mFc Tag)

Catalog No. ABIN7275765

This Recombinant TNFRSF1B protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF1B Protein (AA 23-257) (mFc Tag) (ABIN7275765)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-257

Purification tag / Conjugate: This TNFRSF1B protein is labelled with mFc Tag.

Purpose: Human TNFR2/CD120b/TNFRSF1B Protein

Sequence: Leu23-Asp257

Characteristics: Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu23-Asp257.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).,Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).,Immobilized Human TNFR2, mFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.,Human TNFR2-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human TNFR2 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: TNFR2

Background: Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.

Molecular Weight: 51.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: P20333

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway