Osteoprotegerin Protein (AA 22-401) (His tag)

Catalog No. ABIN7275769

This Recombinant Osteoprotegerin protein is expressed in HEK-293 Cells.

Quick Overview for Osteoprotegerin Protein (AA 22-401) (His tag) (ABIN7275769)

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 22-401

Purification tag / Conjugate: This Osteoprotegerin protein is labelled with His tag.

Purpose: Mouse TNFRSF11B Protein

Sequence: Glu22-Leu401

Characteristics: Recombinant Mouse TNFRSF11B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Leu401.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TNFRSF11B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Alternative Name: TNFRSF11B

Target Type: Chemical

Background: Osteoprotegerin (OPG) is a key regulator in bone metabolism, that also has effect in vascular system. Studies suggest that osteoprotegerin is a critical arterial calcification inhibitor, and is released by endothelial cells as a protective mechanism for their survival in certain pathological conditions, such as diabetes mellitus, chronic kidney disease, and other metabolic disorders.

Molecular Weight: 44.53 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: O08712