TNFRSF12A Protein (AA 28-80) (hFc Tag)

Catalog No. ABIN7275770

Recombinant TNFRSF12A protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF12A Protein (AA 28-80) (hFc Tag) (ABIN7275770)

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-80

Purification tag / Conjugate: This TNFRSF12A protein is labelled with hFc Tag.

Purpose: Mouse TNFRSF12A/TWEAKR Protein

Sequence: Glu28-Per80

Characteristics: Recombinant Mouse TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Glu28-Per80.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.15 nM as determined in SPR assay (Biacore T200).,Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).,Mouse TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TNFRSF12A is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Alternative Name: TNFRSF12A

Background: Tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a) is a receptor for tumor necrosis factor superfamily member 12 (Tnfsf12). Tnfrsf12a is highly inducible and plays a key role in the development of cardiac hypertrophy and heart failure.

Molecular Weight: 32.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q9CR75

Pathways: Apoptosis, Regulation of Cell Size