TNFRSF10A Protein (AA 24-239) (hFc Tag)

Catalog No. ABIN7275776

Recombinant TNFRSF10A protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF10A Protein (AA 24-239) (hFc Tag) (ABIN7275776)

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-239

Purification tag / Conjugate: This TNFRSF10A protein is labelled with hFc Tag.

Purpose: Human TRAIL R1/DR4/TNFRSF10A Protein

Sequence: Ala24-Asn239

Characteristics: Recombinant Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala24-Asn239.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.,Human TRAIL R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TRAIL R1

Background: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis selectively via its interaction with the death receptors TRAILR1/DR4 and TRAILR2/DR5 in a wide range of cancers, while sparing normal cells. Despite its tremendous potential for cancer therapeutics, the translation of TRAIL into the clinic has been confounded by TRAIL-resistant cancer populations.

Molecular Weight: 48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity