TNFRSF10A Protein (AA 34-239) (His-Avi Tag)

Catalog No. ABIN7275777

This Recombinant TNFRSF10A protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF10A Protein (AA 34-239) (His-Avi Tag) (ABIN7275777)

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 34-239

Purification tag / Conjugate: This TNFRSF10A protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein

Sequence: Pro34-Asn239

Characteristics: Recombinant Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro34-Asn239.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TRAIL R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human TRAIL, No Tag-with an affinity constant of 1.02 nM as determined in SPR assay (Biacore T200).,Human TRAIL R1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TRAIL R1

Background: Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells.

Molecular Weight: 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity