DcR2 Protein (AA 56-211) (Biotin,His-Avi Tag)
Quick Overview for DcR2 Protein (AA 56-211) (Biotin,His-Avi Tag) (ABIN7275782)
Target
See all DcR2 (TNFRSF10D) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 56-211
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Purification tag / Conjugate
- This DcR2 protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human TRAIL R4/TNFRSF10D Protein
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Sequence
- Ala56-His211
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Characteristics
- Recombinant Biotinylated Human TRAIL R4/TNFRSF10D Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala56-His211.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Biotinylated Human TRAIL R4, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.135 nM as determined in SPR assay (Biacore T200).,Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test).,Biotinylated Human TRAIL R4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human TRAIL R4 is greater than 95% as determined by SEC-HPLC.
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Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D) (AA 56-211) (Active) protein (His-Avi Tag)
TNFRSF10D Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D) (AA 238-386) protein (His-DYKDDDDK-Strep II Tag)TNFRSF10D Origin: Human Host: HEK-293T Cells Recombinant WB, ELISA, SDS, PI
Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D) (AA 56-187) protein (His-DYKDDDDK-Strep II Tag)TNFRSF10D Origin: Human Host: HEK-293T Cells Recombinant WB, ELISA, SDS, PI
Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D) (AA 56-211) (Active) protein (hIgG-His-tag)TNFRSF10D Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- DcR2 (TNFRSF10D) (Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))
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Alternative Name
- TRAIL R4
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Background
- TNF-related apoptosis inducing ligand (TRAIL) is a potential antitumor protein known for its ability to selectively eliminate various types of tumor cells without exerting toxic effects in normal cells and tissues. TRAIL-R2/DR5 as well as TRAIL-R3/DcR1 and TRAIL-R4/DcR2 were significantly higher expressed in advanced tumour stages.
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Molecular Weight
- 19.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9UBN6
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Pathways
- Apoptosis
Target
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