Tspan-8 Protein (AA 110-205) (hFc Tag)

Catalog No. ABIN7275804

This Recombinant Tspan-8 protein is produced in HEK-293 Cells.

Quick Overview for Tspan-8 Protein (AA 110-205) (hFc Tag) (ABIN7275804)

Target: Tspan-8 (TSPAN8) (Tetraspanin 8 (TSPAN8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 110-205

Purification tag / Conjugate: This Tspan-8 protein is labelled with hFc Tag.

Purpose: Human TSPAN8 Protein

Sequence: Lys110-Asn205

Characteristics: Recombinant Human TSPAN8 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Lys110-Asn205.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TSPAN8, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSPAN8 Antibody, hFc Avi Tag with the EC50 of 35.7ng/ml determined by ELISA.,Human TSPAN8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human TSPAN8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Tspan-8 (TSPAN8) (Tetraspanin 8 (TSPAN8))

Alternative Name: TSPAN8

Background: Tetraspanin 8 (TSPAN8) is a member of the tetraspanin superfamily that forms TSPAN8-mediated protein complexes by interacting with themselves and other various cellular signaling molecules.In physiological conditions, TSPAN8 plays a vital role in the regulation of biological functions, including leukocyte trafficking, angiogenesis and wound repair.

Molecular Weight: 37.30 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.

UniProt: P19075