VEGF121 Protein (AA 27-147) (His-Avi Tag)

Catalog No. ABIN7275825

This Recombinant VEGF121 protein is produced in HEK-293 Cells.

Quick Overview for VEGF121 Protein (AA 27-147) (His-Avi Tag) (ABIN7275825)

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 27-147

Purification tag / Conjugate: This VEGF121 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human VEGF121 Protein

Sequence: Ala27-Arg147

Characteristics: Recombinant Human VEGF121 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg147.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF121, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8 ng/ml determined by ELISA.,Immobilized Human VEGF121, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA.,Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 250 mM Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Alternative Name: VEGF121

Background: Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Molecular Weight: 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: P15692