VSIG4 Protein (AA 25-288) (His tag)

Catalog No. ABIN7275842

Recombinant VSIG4 protein expressed in HEK-293 Cells.

Quick Overview for VSIG4 Protein (AA 25-288) (His tag) (ABIN7275842)

Target: VSIG4 (V-Set and Immunoglobulin Domain-Containing Protein 4 (VSIG4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-288

Purification tag / Conjugate: This VSIG4 protein is labelled with His tag.

Purpose: Cynomolgus VSIG4 Protein

Sequence: Arg25-Ala288

Characteristics: Recombinant Cynomolgus VSIG4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg25-Ala288.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus VSIG4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus VSIG4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VSIG4 (V-Set and Immunoglobulin Domain-Containing Protein 4 (VSIG4))

Alternative Name: VSIG4

Background: VSIG4, a B7 family-related protein, is a negative regulator of T cell activation.T cell activation by APCs is positively and negatively regulated by members of the B7 family. Unlike that of B7 family members, surface expression of VSIG4 was restricted to resting tissue macrophages and absent upon activation by LPS or in autoimmune inflammatory foci. The specific expression of VSIG4 on resting macrophages in tissue suggests that this inhibitory ligand may be important for the maintenance of T cell unresponsiveness in healthy tissues.

Molecular Weight: 30.16 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.

UniProt: A0A7N9D9P6