WISP1 Protein (AA 23-367) (His tag)

Catalog No. ABIN7275850

This Recombinant WISP1 protein is expressed in HEK-293 Cells.

Quick Overview for WISP1 Protein (AA 23-367) (His tag) (ABIN7275850)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 23-367

Purification tag / Conjugate: This WISP1 protein is labelled with His tag.

Purpose: Mouse WISP1 Protein

Sequence: Thr23-Asn367

Characteristics: Recombinant Mouse WISP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr23-Asn367.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse WISP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: WISP1

Background: The interplay between glioma stem cells (GSCs) and the tumor microenvironment plays crucial roles in promoting malignant growth of glioblastoma (GBM), the most lethal brain tumor. WISP1 is preferentially expressed and secreted by GSCs. Silencing WISP1 markedly disrupts GSC maintenance, reduces tumor-supportive TAMs (M2), and potently inhibits GBM growth. WISP1 signals through Integrin α6β1-Akt to maintain GSCs by an autocrine mechanism and M2 TAMs through a paracrine manner.

Molecular Weight: 39.4 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Bis-Tris PAGE result.

UniProt: O54775

Pathways: WNT Signaling, Growth Factor Binding