CD80 Protein (CD80) (Biotin,Fc-Avi Tag)

Catalog No. ABIN7316777

This Recombinant CD80 protein is expressed in HEK-293 Cells.

Quick Overview for CD80 Protein (CD80) (Biotin,Fc-Avi Tag) (ABIN7316777)

Target: CD80

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: 95,00 %

Grade: MALS verified

Product Details

Purification tag / Conjugate: This CD80 protein is labelled with Biotin,Fc-Avi Tag.

Purpose: Biotinylated Human B7-1 / CD80 Protein, Fc,Avitag™ (MALS verified)

Sequence: Val 35 - Asn 242

Characteristics: Biotinylated Human B7-1 / CD80,Fc,Avitag is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).

Endotoxin Level: 1.0 EU per μg

Application Details

Comment:

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl, pH 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: CD80

Alternative Name: B7-1

Background: Synonyms:CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7,Description:B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Molecular Weight: 52.0 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints