CCR2 Protein-VLP (AA 1-360)

Catalog No. ABIN7448153

This VLP CCR2 protein is produced in HEK-293 Cells.

Quick Overview for CCR2 Protein-VLP (AA 1-360) (ABIN7448153)

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Protein Type: VLP

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Immunogen (Imm), Surface Plasmon Resonance (SPR), Functional Studies (Func)

Purity: > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-360

Purpose: Human CCR2b Protein-VLP

Sequence: Met1-Leu360

Characteristics: Recombinant Human CCR2b Protein-VLP is expressed from HEK293.It contains Met1-Leu360.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CCR2b VLP at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CCR2b Antibody, hFc Tag with the EC50 of 12.0ng/ml determined by ELISA.

Application Details

Application Notes:

• Antibody Discovery: Immunization, Screening, Functional Characterization
• Affinity determination: ELISA, SPR
• In vivo pharmacokinetic analysis
• CMC method development
• CAR-T Positive Rate Detection
• Blood sample determination: ELISA

Comment:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS, 300 mM L-Arginine ( pH 7.4). Notice: If you need it for immunization, Do Not use any adjuvant.

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Alternative Name: CCR2b

Background: The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. The actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures.

Molecular Weight: 42.9kDa.

UniProt: P41597-2

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process