CCR8 Protein (AA 1-35) (mFc Tag)

Catalog No. ABIN7454100

Recombinant CCR8 protein expressed in HEK-293 Cells.

Quick Overview for CCR8 Protein (AA 1-35) (mFc Tag) (ABIN7454100)

Target: CCR8 (Chemokine (C-C Motif) Receptor 8 (CCR8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-35

Purification tag / Conjugate: This CCR8 protein is labelled with mFc Tag.

Purpose: Cynomolgus CCR8 Protein

Sequence: Met1-Lys35

Characteristics: Recombinant Cynomolgus CCR8 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Met1-Lys35.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Anti-CCR8 Antibody, hFc Tag captured on CM5 Chip via Anti-Human Fc IgG Antibody can bind Cynomolgus CCR8, mFc Tag with an affinity constant of 0.27 uM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus CCR8, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCR8 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.,Cynomolgus CCR8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus CCR8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CCR8 (Chemokine (C-C Motif) Receptor 8 (CCR8))

Alternative Name: CCR8

Background: CC chemokine receptor (CCR) 8 (previously called CKR-L1 or TER1 and designated CD198), which is expressed on Th2 cells and eosinophils, has been implicated in allergic diseases. CCR8 may regulate monocyte chemotaxis and thymic cell line apoptosis and is alternative coreceptor with CD4 for HIV-1 infection.

Molecular Weight: 29.73 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_015300839