HYAL2 Protein (AA 23-448) (His tag)

Catalog No. ABIN7454133

Recombinant HYAL2 protein expressed in HEK-293 Cells.

Quick Overview for HYAL2 Protein (AA 23-448) (His tag) (ABIN7454133)

Target: HYAL2 (Hyaluronidase-2 (HYAL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-448

Purification tag / Conjugate: This HYAL2 protein is labelled with His tag.

Purpose: Mouse Hyaluronidase 2/HYAL2 Protein

Sequence: Leu23-Asn448

Characteristics: Recombinant Mouse Hyaluronidase 2/HYAL2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asn448.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Hyaluronidase 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Hyaluronidase 2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HYAL2 (Hyaluronidase-2 (HYAL2))

Alternative Name: Hyaluronidase 2

Background: Hyaluronidases, a family of enzymes that are able to degrade hyaluronic acid (HA), are employed in medicine to increase drug diffusion and reverse the effects of HA filler injections. Hyaluronidases are able to dissolve subcutaneous nodules or to correct excessive quantities of injected filler. Upregulation of hyaluronidase 2 (HYAL2), one of somatic hyaluronidase (HAase), was demonstrated in granulation tissue during the healing of equine superficial digital flexor tendon injuries.

Molecular Weight: 49.87 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.

UniProt: O35632

Pathways: Transition Metal Ion Homeostasis, Glycosaminoglycan Metabolic Process