KIT Ligand Protein (KITLG) (AA 26-189)
Quick Overview for KIT Ligand Protein (KITLG) (AA 26-189) (ABIN7454164)
Target
See all KIT Ligand (KITLG) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 26-189
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Purpose
- Human SCF Protein
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Sequence
- Glu26-Ala189
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Characteristics
- Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 0.02 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human SCF at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6μg/ml determined by ELISA. See testing image for detail.
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KIT Ligand (KITLG) (Active) protein
KITLG Origin: Human Host: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Rat Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
KIT Ligand (KITLG) (AA 26-189) (Active) proteinKITLG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, AcA Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- KIT Ligand (KITLG)
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Alternative Name
- SCF
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Background
- Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
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Molecular Weight
- 18.45 kDa same as Tris-Bis PAGE result.
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UniProt
- P21583-1
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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