MUC16 Protein (AA 12783-13467) (His tag)

Catalog No. ABIN7454190

Recombinant MUC16 protein expressed in HEK-293 Cells.

Quick Overview for MUC16 Protein (AA 12783-13467) (His tag) (ABIN7454190)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 12783-13467

Purification tag / Conjugate: This MUC16 protein is labelled with His tag.

Purpose: Human CA125/MUC16 Protein

Sequence: Asp12783-Ser13467

Characteristics: Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp12783-Ser13467.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CA125, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125

Background: MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.

Molecular Weight: 76.17 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.

UniProt: Q8WXI7