VEGF121 Protein (AA 27-147)

Catalog No. ABIN7454231

This Recombinant VEGF121 protein is expressed in HEK-293 Cells.

Quick Overview for VEGF121 Protein (AA 27-147) (ABIN7454231)

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-147

Purpose: Human VEGF121 Protein

Sequence: Ala27-Arg147

Characteristics: Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.,Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.,Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL.,The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Alternative Name: VEGF121

Background: Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Molecular Weight: 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.

UniProt: P15692