HLA-A*03:01 (AA 25-305), (G12V), (Monomer) (Active) protein (His-Avi Tag,KRAS G12V)

Catalog No. ABIN7454294

This Recombinant HLA-A*03:01 protein is expressed in HEK-293 Cells.

Quick Overview for HLA-A*03:01 (AA 25-305), (G12V), (Monomer) (Active) protein (His-Avi Tag,KRAS G12V) (ABIN7454294)

Target: HLA-A*03:01

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-305, G12V, Monomer

Purification tag / Conjugate: His-Avi Tag,KRAS G12V

Purpose: Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein

Sequence: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide

Specificity: Uni-Prot: NP_002107.3 (HLA-A*03:01), P61769 (B2M), VVVGAVGVGK

Characteristics: Recombinant Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody with an affinity constant of 0.11 uM as determined in SPR assay (Biacore T200).,Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag- on Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody with an affinity constant of 11.4 nM as determined in BLI assay (Gator® Prime).,The purity of Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLA-A*03:01

Background: Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

Molecular Weight: 50.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_002107