Nucleolin Protein (NCL) (AA 2-710) (His tag)

Catalog No. ABIN7490643

Recombinant Nucleolin protein expressed in HEK-293 Cells.

Quick Overview for Nucleolin Protein (NCL) (AA 2-710) (His tag) (ABIN7490643)

Target: Nucleolin (NCL)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 2-710

Purification tag / Conjugate: This Nucleolin protein is labelled with His tag.

Purpose: Recombinant human NCL protein with N-terminal 6xHis tag

Specificity: 6xHis tag NCL(Val2-Glu710)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Nucleolin (NCL)

Alternative Name: NCL

Background: C23,Nsr1
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 77.3 kDa after removal of the signal peptide. The apparent molecular mass of His-NCL is 100-130 kDa due to glycosylation.

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization