RNASE4 Protein (AA 30-148) (His tag)

Catalog No. ABIN7490735

This Recombinant RNASE4 protein is expressed in HEK-293 Cells.

Quick Overview for RNASE4 Protein (AA 30-148) (His tag) (ABIN7490735)

Target: RNASE4 (Ribonuclease, RNase A Family, 4 (RNASE4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 30-148

Purification tag / Conjugate: This RNASE4 protein is labelled with His tag.

Purpose: Recombinant mouse RNASE4 protein with C-terminal 6xHis tag

Specificity: Mouse RNASE4(Gln30-Arg148) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: RNASE4 (Ribonuclease, RNase A Family, 4 (RNASE4))

Alternative Name: RNASE4

Background: RNase 4,Rnase4,Ribonuclease 4
This gene encodes a member of the pancreatic ribonuclease A superfamily. The encoded enzyme is sereted and has unique uridine specificity. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the angiogenin, ribonuclease, RNase A family, 5 gene. Each gene splices to a unique downstream exon that contains its complete coding region. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. [provided by RefSeq, Jun 2009]

Molecular Weight: predicted molecular mass of 14.7 kDa after removal of the signal peptide. The apparent molecular mass of mRNASE4-His is 10-15 kDa due to glycosylation.

UniProt: Q9JJH1