HCST Protein (AA 19-48) (Fc Tag)

Catalog No. ABIN7490759

This Recombinant HCST protein is expressed in HEK-293 Cells.

Quick Overview for HCST Protein (AA 19-48) (Fc Tag) (ABIN7490759)

Target: HCST (Hematopoietic Cell Signal Transducer (HCST))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-48

Purification tag / Conjugate: This HCST protein is labelled with Fc Tag.

Purpose: Recombinant human DAP10 protein with C-terminal human Fc tag

Specificity: DAP10(Gln19-Pro48) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: HCST (Hematopoietic Cell Signal Transducer (HCST))

Alternative Name: DAP10

Background: HCST,KAP10,PIK3AP
This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 29.1 kDa after removal of the signal peptide. The apparent molecular mass of DAP10-hFc is 25-55 kDa due to glycosylation.

UniProt: Q9UBK5