HPSE Protein (AA 36-543) (His tag)

Catalog No. ABIN7490767

This Recombinant HPSE protein is produced in HEK-293 Cells.

Quick Overview for HPSE Protein (AA 36-543) (His tag) (ABIN7490767)

Target: HPSE (Heparanase (HPSE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 36-543

Purification tag / Conjugate: This HPSE protein is labelled with His tag.

Purpose: Recombinant human HPSE protein with C-terminal 6xHis tag

Specificity: HPSE(Gln36-Ile543) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: HPSE (Heparanase (HPSE))

Alternative Name: HPSE

Background: Heparanase,Hpa1,HEP,HPA,HPA1,HPR1,HPSE1,HSE1
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Molecular Weight: predicted molecular mass of 58.4 kDa after removal of the signal peptide. The apparent molecular mass of HPSE-His is 55-70 kDa due to glycosylation.

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process