PRNP Protein (AA 90-230) (Fc Tag)

Catalog No. ABIN7490844

This Recombinant PRNP protein is expressed in HEK-293 Cells.

Quick Overview for PRNP Protein (AA 90-230) (Fc Tag) (ABIN7490844)

Target: PRNP (Prion Protein (PRNP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 90-230

Purification tag / Conjugate: This PRNP protein is labelled with Fc Tag.

Purpose: Recombinant human PRNP(90-230) protein with C-terminal human Fc tag

Specificity: PRNP(Gly90-Ser230) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: PRNP (Prion Protein (PRNP))

Alternative Name: PRNP

Background: PrP,ASCR,PrP27-30,PrP33-35C,CD230
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Molecular Weight: predicted molecular mass of 42.2 kDa after removal of the signal peptide. The apparent molecular mass of PRNP(90-230)-hFc is 55-70 kDa due to glycosylation.

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation