ADGRE2 Protein (AA 24-537) (His tag)

Catalog No. ABIN7490879

This Recombinant ADGRE2 protein is expressed in HEK-293 Cells.

Quick Overview for ADGRE2 Protein (AA 24-537) (His tag) (ABIN7490879)

Target: ADGRE2 (Adhesion G protein-coupled Receptor E2 (ADGRE2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 24-537

Purification tag / Conjugate: This ADGRE2 protein is labelled with His tag.

Purpose: Recombinant human ADGRE2 protein with C-terminal 7xHis tag

Specificity: ADGRE2(Gln24-Thr537) 7xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ADGRE2 (Adhesion G protein-coupled Receptor E2 (ADGRE2))

Alternative Name: ADGRE2

Background: Adhesion G protein-coupled receptor E2,EGF-like module receptor 2,EGF-like module-containing mucin-like hormone receptor-like 2,CD312
This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Molecular Weight: predicted molecular mass of 57.2 kDa after removal of the signal peptide. The apparent molecular mass of ADGRE2-His is 70-100 kDa due to glycosylation.

UniProt: Q9UHX3