Asialoglycoprotein Receptor 1 Protein (ASGR1) (AA 62-291) (His tag)

Catalog No. ABIN7491213

Recombinant Asialoglycoprotein Receptor 1 protein expressed in HEK-293 Cells.

Quick Overview for Asialoglycoprotein Receptor 1 Protein (ASGR1) (AA 62-291) (His tag) (ABIN7491213)

Target: Asialoglycoprotein Receptor 1 (ASGR1)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 62-291

Purification tag / Conjugate: This Asialoglycoprotein Receptor 1 protein is labelled with His tag.

Purpose: Recombinant human ASGR1 protein with N-terminal 6xHis tag

Specificity: 6xHis tag ASGR1(Gln62-Leu291)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Asialoglycoprotein Receptor 1 (ASGR1)

Alternative Name: ASGR1

Background: ASGPR,ASGPR1,CLEC4H1,HL-1
This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Molecular Weight: predicted molecular mass of 27.2 kDa after removal of the signal peptide. The apparent molecular mass of His-ASGR1 is 35-55 kDa due to glycosylation.

UniProt: P07306

Pathways: Thyroid Hormone Synthesis