VSIG1 Protein (AA 22-232) (His tag)

Catalog No. ABIN7491437

This Recombinant VSIG1 protein is produced in HEK-293 Cells.

Quick Overview for VSIG1 Protein (AA 22-232) (His tag) (ABIN7491437)

Target: VSIG1 (V-Set and Immunoglobulin Domain Containing 1 (VSIG1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-232

Purification tag / Conjugate: This VSIG1 protein is labelled with His tag.

Purpose: Recombinant Human VSIG1 Protein with C-terminal 6xHis tag

Specificity: VSIG1(Val22-Glu232) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: VSIG1 (V-Set and Immunoglobulin Domain Containing 1 (VSIG1))

Alternative Name: VSIG1

Background: GPA34
This gene encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multiple glycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP) repeats at the C-terminal region. The gene is expressed in normal stomach and testis, as well as in gastric, esophageal and ovarian cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Molecular Weight: predicted molecular mass of 23.7 kDa after removal of the signal peptide. The apparent molecular mass of VSIG1-His is 35-55 kDa due to glycosylation.

UniProt: Q86XK7