CD8A & CD8B (AA 28-196), (Heterodimer) (Active) protein (His tag,DYKDDDDK Tag)

Catalog No. ABIN7504281

This Recombinant CD8A & CD8B protein is produced in HEK-293 Cells.

Quick Overview for CD8A & CD8B (AA 28-196), (Heterodimer) (Active) protein (His tag,DYKDDDDK Tag) (ABIN7504281)

Target: CD8A & CD8B

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-196, Heterodimer

Purification tag / Conjugate: His tag,DYKDDDDK Tag

Purpose: Mouse CD8 alpha&beta Heterodimer Protein

Sequence: Lys28-Tyr196(CD8 alpha) acidic tail & Leu22-Thr175(CD8 beta) basic tail

Specificity: Uni-Prot: P01731 (CD8 alpha), P10300 (CD8 beta)

Characteristics: Recombinant Mouse CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag at the C-terminus of CD8 alpha and Flag tag at the C-terminus of CD8 beta.It contains Lys28-Tyr196(CD8 alpha) acidic tail and Leu22-Thr175(CD8 beta) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD8A & CD8B

Background: CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR.

Molecular Weight: 24.80 kDa (CD8 alpha) and 23.17 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-38 kDa and 42-50 kDa based on Bis-Tris PAGE result.

UniProt: P01731