DKK1 Protein (AA 32-142, N-Term) (mFc Tag)

Catalog No. ABIN7504308

This Recombinant DKK1 protein is produced in HEK-293 Cells.

Quick Overview for DKK1 Protein (AA 32-142, N-Term) (mFc Tag) (ABIN7504308)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-142, N-Term

Purification tag / Conjugate: This DKK1 protein is labelled with mFc Tag.

Purpose: Human DKK1 N terminal Domain Protein

Sequence: Thr32-Asp142

Characteristics: Recombinant Human DKK1 N terminal Domain Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Thr32-Asp142.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human DKK1 N terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human DKK1 N terminal Domain is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

Molecular Weight: 37.35 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation