FSH alpha Protein (AA 25-116) (hFc Tag)

Catalog No. ABIN7504338

This Recombinant FSH alpha protein is produced in HEK-293 Cells.

Quick Overview for FSH alpha Protein (AA 25-116) (hFc Tag) (ABIN7504338)

Target: FSH alpha (Follicle-Stimulating Hormone alpha)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-116

Purification tag / Conjugate: This FSH alpha protein is labelled with hFc Tag.

Purpose: Human FSH alpha&beta Protein

Sequence: Ala25-Ser116 (FSH alpha) & Asn19-Glu129 (FSH beta)

Specificity: Uni-Prot: P01215 (FSH alpha), P01225 (FSH beta)

Characteristics: Recombinant Human FSH alpha&beta Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of FSH beta.It contains Ala25-Ser116 (FSH alpha) & Asn19-Glu129 (FSH beta).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human FSH alpha&beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human FSH alpha&beta is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FSH alpha (Follicle-Stimulating Hormone alpha)

Alternative Name: FSH alpha

Background: FSH (Follicle-stimulating hormone) is a gonadotropin, a glycoprotein polypeptide hormone. It has a functionally indispensable 96 amino acid α subunit that is common to LH, TSH and hCG, in addition to a structurally unique β subunit. FSH is synthesized and secreted by the gonadotropic cells of the anterior pituitary gland and regulates the development, growth, pubertal maturation, and reproductive processes of the body.

Molecular Weight: 10.20 kDa (FSH alpha) and 39.2 kDa (FSH beta). Due to glycosylation, the protein migrates to 25-30 kDa (FSH alpha) and 50-60 kDa (FSH beta) based on Bis-Tris PAGE result.

UniProt: P01215