ITCH Protein (AA 485-862)

Catalog No. ABIN7504453

This Recombinant ITCH protein is expressed in Escherichia coli (E. coli).

Quick Overview for ITCH Protein (AA 485-862) (ABIN7504453)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 485-862

Purpose: Human ITCH Protein

Sequence: Arg485-Glu862

Characteristics: Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human ITCH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ITCH is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background: Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.

Molecular Weight: 44.98 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_113671

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events