LY6G6D Protein (AA 20-104) (His tag)

Catalog No. ABIN7504463

Recombinant LY6G6D protein expressed in HEK-293 Cells.

Quick Overview for LY6G6D Protein (AA 20-104) (His tag) (ABIN7504463)

Target: LY6G6D (Lymphocyte Antigen 6 Complex, Locus G6D (LY6G6D))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 20-104

Purification tag / Conjugate: This LY6G6D protein is labelled with His tag.

Purpose: Human LY6G6D Protein

Sequence: Asn20-Ser104

Characteristics: Recombinant Human LY6G6D Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asn20-Ser104.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LY6G6D, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.,Human LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-LY6G6D Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human LY6G6D, His Tag with an affinity constant of 2.48 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LY6G6D (Lymphocyte Antigen 6 Complex, Locus G6D (LY6G6D))

Alternative Name: LY6G6D

Background: LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager.LY6G6D was identified as a selectively expressed CRC antigen that can be utilized to potently re-direct and activate cytotoxic T-cells to lyse LY6G6D expressing CRC using a TcE. This effect can be spread to target negative neighboring tumor cells, potentially leading to improved therapeutic efficacy.

Molecular Weight: 10.20 kDa. Due to glycosylation, the protein migrates to 13-20 kDa based on Bis-Tris PAGE result.

UniProt: O95868