LC3B Protein (AA 1-120) (His tag)

Catalog No. ABIN7505007

Recombinant LC3B protein expressed in Escherichia coli (E. coli).

Quick Overview for LC3B Protein (AA 1-120) (His tag) (ABIN7505007)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-120

Purification tag / Conjugate: This LC3B protein is labelled with His tag.

Sequence: Met1-Gly120

Characteristics: A DNA sequence encoding theHuman MAP1LC3B protein (Q9GZQ8) (Met1-Gly120) was expressed with a N-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: MAP1LC3B

Background:

Abbreviation: MAP1LC3B

Target Synonym: Autophagy-related protein LC3 B,Autophagy-related ubiquitin-like modifier LC3 B,MAP1 light chain 3-like protein 2,MAP1A/MAP1B light chain 3 B,MAP1A/MAP1B LC3 B,Microtubule-associated protein 1 light chain 3 beta

Background: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes).Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria.While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy.

Molecular Weight:

Calculated MW: 14.1 kDa

Observed MW: 18 kDa

UniProt: Q9GZQ8

Pathways: Autophagy