LC3B Protein (AA 1-125) (GST tag)
(3 references)-
- Target See all LC3B (MAP1LC3B) Proteins
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-125
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This LC3B protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- MAP1LC3B (Human) Recombinant Protein (P01)
- Sequence
- MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG MKLSV
- Characteristics
- Human MAP1LC3B full-length ORF ( AAH18634, 1 a.a. - 125 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-140) protein (His tag)
MAP1LC3B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-107) protein (His-IF2DI Tag)MAP1LC3B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-71) protein (GST tag)MAP1LC3B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 2-120) protein (His tag)Crystallography grade MAP1LC3B Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 2-120) protein (His tag)Crystallography grade MAP1LC3B Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." in: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, (2014) (PubMed).
: "Immunohistochemical analysis of macroautophagy: recommendations and limitations." in: Autophagy, Vol. 9, Issue 3, pp. 386-402, (2013) (PubMed).
: "LC3 immunostaining pitfalls." in: Histopathology, Vol. 62, Issue 6, pp. 962-3, (2013) (PubMed).
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: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." in: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, (2014) (PubMed).
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- Target
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
- Alternative Name
- MAP1LC3B (MAP1LC3B Products)
- Synonyms
- ATG8F Protein, LC3B Protein, MAP1A/1BLC3 Protein, MAP1LC3B-a Protein, Map1lc3 Protein, Mpl3 Protein, zbs559 Protein, 1010001C15Rik Protein, Atg8 Protein, LC3b Protein, zgc:56434 Protein, wu:fb60g11 Protein, map1lc3b Protein, MGC76283 Protein, MAP1LC3B Protein, microtubule associated protein 1 light chain 3 beta Protein, microtubule-associated protein 1 light chain 3 beta Protein, microtubule associated protein 1 light chain 3 beta L homeolog Protein, MAP1LC3B Protein, Map1lc3b Protein, map1lc3b Protein, map1lc3b.L Protein
- Background
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Full Gene Name: microtubule-associated protein 1 light chain 3 beta
Synonyms: LC3B,MAP1A/1BLC3 - Gene ID
- 81631
- Pathways
- Autophagy
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