UCP1 Protein (AA 1-307) (His tag)

Catalog No. ABIN7505050

Product Details

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-307

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UCP1 protein is labelled with His tag.

Sequence: Met 1-Thr 307

Characteristics: A DNA sequence encoding the Human UCP1 protein (P25874) (Met 1-Thr 307) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP1 (UCP1 Products)

Background:

Abbreviation: UCP1

Target Synonym: UCP1,SLC25A7,UCP

Background: Mitochondrial brown fat uncoupling protein 1 (UCP1, also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

Molecular Weight:

Calculated MW: 33.66 kDa

Observed MW: 31 kDa

UniProt: P25874

Pathways: Proton Transport, Brown Fat Cell Differentiation