MGEA5 Protein (AA 6-240) (His tag)
Quick Overview for MGEA5 Protein (AA 6-240) (His tag) (ABIN7505107)
Target
See all MGEA5 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 6-240
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Purification tag / Conjugate
- This MGEA5 protein is labelled with His tag.
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Sequence
- Ser 6-Lys 240
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Characteristics
- A DNA sequence encoding the Human OGA protein (O60502) (Ser 6-Lys 240) was expressed with a N-His tag.
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Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-916) protein (His tag)
custom-made MGEA5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-916) protein (His tag)custom-made MGEA5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-209) protein (His-IF2DI Tag)MGEA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-916) protein (GST tag)MGEA5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)MGEA5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))
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Alternative Name
- OGA
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Background
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Abbreviation: OGA
Target Synonym: OGA,HEXC,KIAA0679,MEA5,MGEA5,Protein O-GlcNAcase
Background: Isoform 1:Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3 :Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
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Molecular Weight
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Calculated MW: 25.74 kDa
Observed MW: 25 kDa
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UniProt
- O60502
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Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process
Target
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