CASR Protein (AA 863-1078) (His tag)

Catalog No. ABIN7505157

Recombinant CASR protein expressed in Escherichia coli (E. coli).

Quick Overview for CASR Protein (AA 863-1078) (His tag) (ABIN7505157)

Target: CASR (Calcium-Sensing Receptor (CASR))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 863-1078

Purification tag / Conjugate: This CASR protein is labelled with His tag.

Sequence: Lys 863-Ser 1078

Characteristics: A DNA sequence encoding the Human CaSR protein (P41180) (Lys 863-Ser 1078) was expressed with N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CASR (Calcium-Sensing Receptor (CASR))

Alternative Name: CaSR

Background:

Abbreviation: CaSR

Target Synonym: Ca sensing receptor,Ca2+ sensing receptor 1,CAR,EIG8,Extracellular calcium sensing receptor,FHH,FIH,GPRC2A,HHC1,NSHPT,PCAR 1,PCaR1

Background: CaSR, the extracellular Calcium-Sensing Receptor, is a widely expressed G-protein coupled receptor (GPCR) involved in calcium homeostasis. CaSR operates as a sensor in parathyroid and kidney, and alterations in its activity have been shown to cause thyroid disease in humans. Activation of the receptor in response to extracellular calcium or other ligands causes activation of phospholipase C (PLC), release of IP3 and release of calcium from intracellular stores. Proinflammatory cytokines IL-1β and TNF-α increase CaSR gene expression in human thyroid and kidney cells through activation of the NF-κB pathway, and this pathway may be involved in hypocalcemia often seen in critically ill patients. Elevated calcium concentration and CaSR expression have been linked to proliferation and metastasis of skeletal metastatic prostate cancer cell lines. In intestinal epithelial cells, CaSR is involved in regulation of cyclic nucleotide metabolism and the fluid secretion that results in life-threatening fluid loss in response to intestinal pathogens. The interaction of CaSR with the actin-binding protein filamin may provide scaffolding for the organization of signaling pathways converging on the cytoskeleton, including CaSR-mediated MAPK pathway activation.

Molecular Weight:

Calculated MW: 23.65 kDa

Observed MW: 35 kDa

UniProt: P41180

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis