Glypican 3 Protein (GPC3) (AA 408-561) (His tag)

Catalog No. ABIN7505210

This Recombinant Glypican 3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Glypican 3 Protein (GPC3) (AA 408-561) (His tag) (ABIN7505210)

Target: Glypican 3 (GPC3)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 408-561

Purification tag / Conjugate: This Glypican 3 protein is labelled with His tag.

Sequence: Tyr 408-Pro 561

Characteristics: A DNA sequence encoding the Human GPC3 protein (P51654) (Tyr 408-Pro 561) was expressed with N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background:

Abbreviation: GPC3

Target Synonym: DGSX,Glypican 3,GTR2-2,MXR7,OCI-5,SDYS,SGB,SGBS,SGBS1

Background: Glypican-3/GPC3 is a member of the glypican family. It belongs to the glypican family and is highly expressed in lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceeds both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.

Molecular Weight:

Calculated MW: 16.83 kDa

Observed MW: 23 kDa

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process