Vinculin Protein (VCL) (AA 1-650) (His tag)

Catalog No. ABIN7505228

This Recombinant Vinculin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Vinculin Protein (VCL) (AA 1-650) (His tag) (ABIN7505228)

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-650

Purification tag / Conjugate: This Vinculin protein is labelled with His tag.

Sequence: Met 1-Val650

Characteristics: A DNA sequence encoding the Human Vinculin(VCL) protein (P18206) (Met 1-Val650) was expressed with a N-His&C-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background:

Abbreviation: Vinculin,VCL

Target Synonym: Vinculin,Metavinculin,VCL,CMD1W,CMH15,HEL114,MV,MVCL

Background: Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/α-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.

Molecular Weight:

Calculated MW: 71.39 kDa

Observed MW: 60 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2