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EIF4E Protein (AA 2-217) (GST-His-Tag)

This Recombinant EIF4E protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN7505312

Quick Overview for EIF4E Protein (AA 2-217) (GST-His-Tag) (ABIN7505312)

Target

See all EIF4E Proteins
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type

Recombinant

Origin

  • 6
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Human

Source

  • 5
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Escherichia coli (E. coli)

Purity

> 95 % as determined by reducing SDS-PAGE.
  • Protein Characteristics

    AA 2-217

    Purification tag / Conjugate

    This EIF4E protein is labelled with GST-His-Tag.

    Sequence

    Ala 2-Val 217

    Characteristics

    A DNA sequence encoding the Human eIF4E protein (P06730) (Ala 2-Val 217) was expressed with a N-GST&C-His tag.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Lyophilized from sterile PBS, pH 7.4.
    Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

    Expiry Date

    12 months
  • Target

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    Alternative Name

    eIF4E

    Background

    Abbreviation: eIF4E

    Target Synonym: Eukaryotic translation initiation factor 4E,eIF-4E,eIF-4F 25 kDa subunit,mRNA cap-binding protein,EIF4E,EIF4EL1,EIF4F

    Background: Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

    Molecular Weight

    Calculated MW: 48.65 kDa

    Observed MW: 52 kDa

    UniProt

    P06730

    Pathways

    BCR Signaling
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