DNMT1 Protein (AA 1396-1583) (His tag)

Catalog No. ABIN7505354

This Recombinant DNMT1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for DNMT1 Protein (AA 1396-1583) (His tag) (ABIN7505354)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1396-1583

Purification tag / Conjugate: This DNMT1 protein is labelled with His tag.

Sequence: Leu1396-Pro1583

Characteristics: A DNA sequence encoding the Mouse DNMT1 (P13864-1) (Leu1396-Pro1583) was expressed with a polyhistidine tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background:

Abbreviation: DNMT1

Target Synonym: DNA (cytosine-5)-methyltransferase 1,Dnmt1,Met-1,DNA methyltransferase MmuI,DNA MTase MmuI,M.MmuI,MCMT,Dnmt,Met1,Uim

Background: Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.

Molecular Weight:

Calculated MW: 21.46 kDa

Observed MW: 23.76 kDa

UniProt: P13864-1

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection