MCAM Protein (AA 341-563) (His tag)

Catalog No. ABIN7505436

Recombinant MCAM protein expressed in Escherichia coli (E. coli).

Quick Overview for MCAM Protein (AA 341-563) (His tag) (ABIN7505436)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 341-563

Purification tag / Conjugate: This MCAM protein is labelled with His tag.

Sequence: Leu 341-Val 563

Characteristics: A DNA sequence encoding the Mouse CD146/MCAM protein (Q8R2Y2) (Leu 341-Val 563) was expressed with N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background:

Abbreviation: CD146,MCAM

Target Synonym: 1-gicerin,AV025631,CD146,CD149,Muc18,s-endo,s-gicerin

Background: The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

Molecular Weight:

Calculated MW: 24.42 kDa

Observed MW: 35 kDa

UniProt: Q8R2Y2