PDGFB Protein (AA 74-182) (His tag)

Catalog No. ABIN7505479

Product Details

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Protein Type: Recombinant

Protein Characteristics: AA 74-182

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PDGFB protein is labelled with His tag.

Sequence: Ser74-Thr182

Characteristics: A DNA sequence encoding the Rat PDGF-B (Q05028-1) (Ser74-Thr182) was expressed with a polyhistidine tag at the N-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGF-B (PDGFB Products)

Background:

Abbreviation: PDGF-B

Target Synonym: Becaplermin,PDGF 2,PDGF B chain,PDGF subunit B,PDGF-2,PDGF2,Pdgfb,PDGFB,Platelet derived growth factor 2,Platelet-derived growth factor B chain,Platelet-derived growth factor beta polypeptide,Platelet-derived growth factor subunit B,Proto-oncogene c-Sis,SIS,SSV

Background: The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Molecular Weight:

Calculated MW: 12.2 kDa

Observed MW: 14.5 kDa

UniProt: Q05028-1

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling