DC-SIGN/CD209 Protein (His tag)

Catalog No. ABIN7535683

This Recombinant DC-SIGN/CD209 protein is expressed in HEK-293 Cells.

Quick Overview for DC-SIGN/CD209 Protein (His tag) (ABIN7535683)

Target: DC-SIGN/CD209 (CD209) (CD209)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This DC-SIGN/CD209 protein is labelled with His tag.

Purpose: Active Recombinant Human DC-SIGN/CD209 Protein

Sequence: QVSKVPSSIS QEQSRQDAIY QNLTQLKAAV GELSEKSKLQ EIYQELTQLK AAVGELPEKS KLQEIYQELT RLKAAVGELP EKSKLQEIYQ ELTWLKAAVG ELPEKSKMQE IYQELTRLKA AVGELPEKSK QQEIYQELTR LKAAVGELPE KSKQQEIYQE LTRLKAAVGE LPEKSKQQEI YQELTQLKAA VERLCHPCPW EWTFFQGNCY FMSNSQRNWH DSITACKEVG AQLVVIKSAE EQNFLQLQSS RSNRFTWMGL SDLNQEGTWQ WVDGSPLLPS FKQYWNRGEP NNVGEEDCAE FSGNGWNDDK CNLAKFWICK KSAASCSRDE EQFLSPAPAT PNPPPA

Specificity: Gln59-Ala404

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Human DC_x001e_SIGN/CD209 Protein coated plates (5 μg/mL with 100 μL/well), approximately 10-20% of added cells will adhere after 1 hour at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: DC-SIGN/CD209

Background:

Description: Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.

Name: CD209,CDSIGN,CLEC4L,DC-SIGN,DC-SIGN1, CLEC4L, DC-SIGN, DC-SIGN1

Gene ID: 30835

UniProt: Q9NNX6-1