CD86 Protein (CD86) (Fc Tag)

Catalog No. ABIN7519817

This Recombinant CD86 protein is expressed in HEK-293 Cells.

Quick Overview for CD86 Protein (CD86) (Fc Tag) (ABIN7519817)

Target: CD86

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 97 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD86 protein is labelled with Fc Tag.

Purpose: Active Recombinant Mouse B7-2/CD86 Protein

Sequence: VSVETQAYFN GTAYLPCPFT KAQNISLSEL VVFWQDQQKL VLYEHYLGTE KLDSVNAKYL GRTSFDRNNW TLRLHNVQIK DMGSYDCFIQ KKPPTGSIIL QQTLTELSVI ANFSEPEIKL AQNVTGNSGI NLTCTSKQGH PKPKKMYFLI TNSTNEYGDN MQISQDNVTE LFSISNSLSL SFPDGVWHMT VVCVLETESM KISSKPLNFT QEFPSPQTYW KE

Specificity: Val24-Glu245

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse CTLA-4/CD152 at 2 μg/mL (100 μL/well) can bind Mouse B7-2/CD86 with a linear range of 0.1-54.9 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: CD86

Alternative Name: B7-2/CD86

Background:

Description: CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. CD86 is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Name: CD86,B7-2,B70,CD28LG2,LAB72,MGC34413,CD86,CD86,B7-2,B70,CD28LG2,LAB72,MGC34413,CD86

Gene ID: 12524

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation