IGFBP4 Protein (His tag)

Catalog No. ABIN7520090

This Recombinant IGFBP4 protein is expressed in HEK-293 Cells.

Quick Overview for IGFBP4 Protein (His tag) (ABIN7520090)

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 92 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This IGFBP4 protein is labelled with His tag.

Purpose: Active Recombinant Human IGFBP-4 Protein

Sequence: MLPLCLVAAL LLAAGPGPSL GDEAIHCPPC SEEKLARCRP PVGCEELVRE PGCGCCATCA LGLGMPCGVY TPRCGSGLRC YPPRGVEKPL HTLMHGQGVC MELAEIEAIQ ESLQPSDKDE GDHPNNSFSP CSAHDRRCLQ KHFAKIRDRS TSGGKMKVNG APREDARPVP QGSCQSELHR ALERLAASQS RTHEDLYIIP IPNCDRNGNF HPKQCHPALD GQRGKCWCVD RKTGVKLPGG LEPKGELDCH QLADSFRE

Specificity: Met1-Glu258

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 at 1 μg/mL (100 μL/well) can bind Human IGF1 with a linear range of 1.95-111.32 ng/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 Protein at 1 μg/mL (100 μL/well) can bind IGF1 with a linear range of 1.95-122.3ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Alternative Name: IGFBP-4

Background:

Description: IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins . IGFBP-4 binds both insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. It circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. There is mounting evidence that IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

Name: IGFBP4,BP-4,HT29-IGFBP,IBP4,IGFBP-4

Gene ID: 3487

UniProt: P22692

Pathways: WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process