JAM3 Protein (Ala149Pro) (Fc Tag,His tag)

Catalog No. ABIN7534135

Product Details

Target: JAM3 (Junctional Adhesion Molecule 3 (JAM3))

Protein Type: Recombinant

Protein Characteristics: Ala149Pro

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This JAM3 protein is labelled with Fc Tag,His tag.

Purpose: Recombinant Human JAM-3(A149P) Protein

Sequence: VNLKSSNRTP VVQEFESVEL SCIITDSQTS DPRIEWKKIQ DEQTTYVFFD NKIQGDLAGR AEILGKTSLK IWNVTRRDSA LYRCEVVARN DRKEIDEIVI ELTVQVKPVT PVCRVPKPVP VGKMATLHCQ ESEGHPRPHY SWYRNDVPLP TDSRANPRFR NSSFHLNSET GTLVFTAVHK DDSGQYYCIA SNDAGSARCE EQEMEVYDLN

Specificity: Val32-Asn241(A149P)

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: JAM3 (Junctional Adhesion Molecule 3 (JAM3))

Alternative Name: JAM-3 (JAM3 Products)

Synonyms: JAM3 Protein, jam3 Protein, sr:nyz155 Protein, wu:fb30h11 Protein, wu:fc08c06 Protein, wu:fc13e04 Protein, wu:fc25g11 Protein, jamc.2 Protein, 1110002N23Rik Protein, JAM-3 Protein, JAM-C Protein, Jcam3 Protein, JAM-2 Protein, JAMC Protein, junctional adhesion molecule 3 Protein, junctional adhesion molecule 3b Protein, junctional adhesion molecule 3a Protein, junction adhesion molecule 3 Protein, JAM3 Protein, jam3b Protein, jam3a Protein, Jam3 Protein

Background:

Description: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.

Name: JAM3,JAM-2,JAM-3,JAM-C,JAMC

Gene ID: 83700

UniProt: Q9BX67