GPR31 Protein
Quick Overview for GPR31 Protein (ABIN7538269)
Target
See all GPR31 ProteinsProtein Type
Origin
Source
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Purpose
- Human GPR31 full length protein-synthetic nanodisc
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Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (His tag)
custom-made GPR31 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (His tag)custom-made GPR31 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (GST tag)GPR31 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- GPR31 (G Protein-Coupled Receptor 31 (GPR31))
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Alternative Name
- GPR31
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Background
- High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.[UniProtKB/Swiss-Prot Function]
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Molecular Weight
- The human full length GPR31 protein has a MW of 35.1kDa
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UniProt
- O00270
Target
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