MUC16 Protein (AA 13810-14451) (His tag)

Catalog No. ABIN7538396

This Recombinant MUC16 protein is produced in Mammalian Cells.

Quick Overview for MUC16 Protein (AA 13810-14451) (His tag) (ABIN7538396)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 13810-14451

Purification tag / Conjugate: This MUC16 protein is labelled with His tag.

Purpose: Recombinant human MUC16(13810-14451) Protein with C-terminal 10xHis tag

Specificity: MUC16 (Pro13810-Pro14451) 10xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: MUC16

Background: This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes. [provided by RefSeq, May 2017]

Molecular Weight: predicted molecular mass of 73.2 kDa after removal of the signal peptide. The apparent molecular mass of MUC16(13810-14451)-His is 100-130 kDa due to glycosylation.

UniProt: Q8WXI7